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Run Types for TruSeq v3 Chemistry

HiSeq 2500 System User Guide

Run Types for TruSeq v3 Chemistry

The following table shows types of sequencing runs and the number of possible cycles for

each read when using TruSeq v3 chemistry. Use this information as a reference when

setting up the run.

Run Type Read 1

Cycles

Index 1 (i7)

Read Cycles

Index 2 (i5)

Read Cycles

Cycles

Total

Cycles

Single-Read,

Non-Indexed

≤ 101 -- -- -- ≤ 101

Single-Read,

Single-Indexed

≤ 101 6 or 7 ¹

8 ²

-- -- ≤ 108 ¹

≤ 109 ²

Single-Read,

Dual-Indexed

≤ 101 8 8 -- ≤ 117

Paired-End,

Non-Indexed

≤ 101 -- -- ≤ 101 ≤ 202

Paired-End,

Single-Indexed

≤ 101 7 ¹

8 ²

-- ≤ 101 ≤ 209 ¹

≤ 210 ²

Paired-End,

Dual-Indexed

≤ 101 8 7 + 8 ³ ≤ 101 ≤ 225

¹ Number of cycles for single-indexed libraries

² Number of cycles for dual-indexed libraries

³ Index 2 Read of a paired-end dual-indexed run includes 7 additional chemistry-only cycles

1 2 ... 50 51 52 53 54 55 56 57 58 59 60 ... 153 154

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