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HiSeq 2500 System User Guide
ix
Table of Contents
Revision History vii
Table of Contents ix
Chapter 1 Overview 1
Introduction 2
HiSeq 2500 Components 3
Start the HiSeq 2500 6
HiSeq 2500 Software 7
Available Disk Space 12
Sample Sheet Overview 13
Sequencing Consumables 14
Additional Resources 16
Chapter 2 Perform a HiSeq v4 Run 17
Introduction 18
HiSeq v4 Sequencing Workflow 19
Run Types for HiSeq v4 Chemistry 20
Enter Run Parameters 21
Load and Prime Reagents 26
Load a Flow Cell 35
Monitor the Run 40
Chapter 3 Perform a TruSeq v3 Run 41
Introduction 42
TruSeq v3 Sequencing Workflow 43
Run Types for TruSeq v3 Chemistry 45
Enter Run Parameters 46
Load and Prime Reagents 51
Load a Flow Cell 59
Monitor the Run 64
Prepare Reagents for Read 2 65
Load Reagents for Read 2 66
Chapter 4 Perform a Rapid Run 69
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